Journal: Biology Methods & Protocols

Article Title: PrimerPooler: automated primer pooling to prepare library for targeted sequencing

doi: 10.1093/biomethods/bpx006

Figure Lengend Snippet: Comparison of available tools for constructing libraries for next-generation sequencing

Article Snippet: Two DNA samples from FFPE tissues were subjected to preamplification, Fluidigm Access Array multiplex PCR and Illumina MiSeQ sequencing based on the primer combination suggested by the above PrimerPooler analyses.

Techniques: Comparison, Multiplex Assay, Control

Journal: Biology Methods & Protocols

Article Title: PrimerPooler: automated primer pooling to prepare library for targeted sequencing

doi: 10.1093/biomethods/bpx006

Figure Lengend Snippet: Coverage of targeted sequence regions by Fluidigm Access Array multiplex PCR and Illumina MiSeQ sequencing. Two FFPE DNA samples were tested under various experimental conditions including 50- and 75-ng DNA for pre-amplication, and 1l of 2.5- and 5.0-fold diluted pre-amplified products for Fluidigm Access Array multiplex PCR. An average of 3269 reads was achieved for the targeted regions, with 95% of targets covered by at least 50 reads, the minimal depth of reads for confident variant calling.

Article Snippet: Two DNA samples from FFPE tissues were subjected to preamplification, Fluidigm Access Array multiplex PCR and Illumina MiSeQ sequencing based on the primer combination suggested by the above PrimerPooler analyses.

Techniques: Sequencing, Multiplex Assay, Amplification, Variant Assay